5A5L
Structure of dual function FBPase SBPase from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 143.090, 143.090, 76.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.416 - 2.340 |
| R-factor | 0.1685 |
| Rwork | 0.166 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r8t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.050 | 0.630 |
| Number of reflections | 17040 | |
| <I/σ(I)> | 9.96 | 1.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.51 | 8.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.06 M NAHEPES (PH 7.5), 0.12 M MGCL2, 27% V/V PEG 400 |
