5A5K
AtGSTF2 from Arabidopsis thaliana in complex with camalexin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.100, 113.720, 132.020 |
| Unit cell angles | 83.65, 79.47, 65.85 |
Refinement procedure
| Resolution | 87.580 - 2.770 |
| R-factor | 0.25207 |
| Rwork | 0.250 |
| R-free | 0.28428 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.913 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.580 | 2.840 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.100 | 0.720 |
| Number of reflections | 126932 | |
| <I/σ(I)> | 6.8 | 1.8 |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2 M SODIUM ACETATE AND 20% (W/V) PEG 3350; PH 7.0; PROTEIN AT 10 MG PER ML |
