5A59
The structure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with T-antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 87.200, 121.890, 139.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.740 - 2.500 |
| R-factor | 0.16963 |
| Rwork | 0.167 |
| R-free | 0.22875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.507 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.160 | 0.500 |
| Number of reflections | 49388 | |
| <I/σ(I)> | 6.2 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
