5A51
The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and phophatidyl serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.583, 89.769, 109.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.787 - 1.602 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v29 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 1.720 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 88958 | |
| <I/σ(I)> | 14.2 | 6.7 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 13.8 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | FROM 20 TO 25 % OF POLYETHYLENE GLYCOL 6K, 0.1M MES PH 6.0 TO 6.5 AND 0.1M LICL 5MM PSF |
