5A50
The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions, Zn and Phopho Choline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.737, 89.150, 112.475 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.564 - 2.400 |
R-factor | 0.1971 |
Rwork | 0.195 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v29 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.227 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.520 |
Number of reflections | 27044 | |
<I/σ(I)> | 14.2 | 3.3 |
Completeness [%] | 99.2 | 99 |
Redundancy | 6.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20 TO 25 % OF POLYETHYLENE GLYCOL 6K, 0.1M MES PH 6.0 TO 6.5 ZNCL2 5MM POC |