5A4W
AtGSTF2 from Arabidopsis thaliana in complex with quercetrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-05-22 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.350, 93.570, 152.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.090 - 2.250 |
| R-factor | 0.20895 |
| Rwork | 0.207 |
| R-free | 0.24195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.730 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.090 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.720 |
| Number of reflections | 60022 | |
| <I/σ(I)> | 16.5 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M PTPC (PROPIONIC ACID, CACODYLATE, BIS-TRIS PROPANE SYSTEM), 15% PEG 1.5K; 10 MG PER ML PROTEIN, pH 7 |
