5A4V
AtGSTF2 from Arabidopsis thaliana in complex with quercetin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-05-22 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.030, 94.830, 153.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.590 - 2.380 |
| R-factor | 0.20483 |
| Rwork | 0.203 |
| R-free | 0.24472 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.685 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.090 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.110 | 0.680 |
| Number of reflections | 52158 | |
| <I/σ(I)> | 14.9 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M PTCP (PROPIONIC ACID, CACODYLATE, BIS-TRIS PROPANE SYSTEM), 15% PEG 1.5K, PROTEIN AT 10 MG PER ML, pH 7 |
