5A4O
Crystal structure of BPSL1147, a PC4 homolog from Burkholderia pseudomallei K96243 (orthorhombic crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.374, 50.149, 63.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.360 - 1.760 |
| R-factor | 0.19614 |
| Rwork | 0.191 |
| R-free | 0.24092 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TETRAGONAL CRYSTAL FORM OF THE SAME PROTEIN HOMODIMER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.120 | 1.020 |
| Number of reflections | 12701 | |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | 20 MM TRIS/HCL PH 9.0, 600 MM KCL, TEMPERATURE 277 K |
