5A49

Crystal structure of the LOTUS domain (aa 139-222) of Drosophila Oskar in C222

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Spacegroup name	C 2 2 2
Unit cell lengths	134.060, 198.630, 89.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.359 - 2.098
R-factor	0.1945
Rwork	0.194
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.742
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Rmerge	0.060
Number of reflections	69144
<I/σ(I)>	15.1
Completeness [%]	99.1
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1