5A48

Crystal structure of the LOTUS domain (aa 139-240) of Drosophila Oskar in P65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65
Unit cell lengths	53.160, 53.160, 109.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.038 - 2.350
R-factor	0.1882
Rwork	0.186
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.710
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Rmerge	0.230
Number of reflections	7333
<I/σ(I)>	9.66
Completeness [%]	99.9
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1