5A42
Cryo-EM single particle 3D reconstruction of the native conformation of E. coli alpha-2-macroglobulin (ECAM)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM CPC |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI EAGLE (4k x 4k) |
| Number of Images | 130 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 16 |
| Software | UCSF Chimera,EMAN,Xmipp |
| Symmetry | C1 |
