5A3V
Crystal structure of the chloroplastic gamma-ketol reductase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.720, 142.650, 209.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.267 - 2.340 |
| R-factor | 0.1849 |
| Rwork | 0.181 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.267 | 2.490 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.134 | 0.471 |
| Number of reflections | 28266 | |
| <I/σ(I)> | 13.24 | 2.69 |
| Completeness [%] | 97.6 | 87.6 |
| Redundancy | 6.64 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM ACETATE, 0.1 M TRIS-HCL PH 8.5, 32% PEG4000 (V/V) |
