5A3F

Crystal structure of the dynamin tetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-03
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	97.696, 98.002, 401.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.465 - 3.700
R-factor	0.2339
Rwork	0.232
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2AKA 3snh 1dyn
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.470	3.800
High resolution limit [Å]	3.700	3.700
Rmerge	0.070	1.300
Number of reflections	42081
<I/σ(I)>	16.6	1.83
Completeness [%]	99.6	99.3
Redundancy	7.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		15% MPD, 100MM MES/NAOH (PH 6.5)