5A3E

2.5A structure of lysozyme determined by MicroED with data from a single crystal

Experimental procedure

Temperature [K]	100
Collection date	2014-02-05
Spacegroup name	P 43 21 2
Unit cell lengths	75.890, 75.890, 36.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.189 - 2.501
R-factor	0.2152
Rwork	0.213
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3j6k
RMSD bond length	0.003
RMSD bond angle	0.687
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	11.200	2.600
High resolution limit [Å]	2.500	2.500
Rmerge		0.292
Number of reflections	3116
Completeness [%]	80.1	80.1
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.5