5A35
Crystal structure of Glycine Cleavage Protein H-Like (GcvH-L) from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.123, 64.123, 73.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.530 - 1.500 |
| R-factor | 0.15395 |
| Rwork | 0.153 |
| R-free | 0.17257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onl |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.530 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.570 |
| Number of reflections | 28124 | |
| <I/σ(I)> | 27.1 | 2.9 |
| Completeness [%] | 99.3 | 93 |
| Redundancy | 8.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.0 M NAH2PO4/K2HPO4 PH 7.0 |
