5A2P

THE COMPLEX STRUCTURE OF PDZ DOMAINS IN SYNTENIN-1 WITH 4L PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	93
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43
Unit cell lengths	64.200, 64.200, 201.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.903 - 2.496
R-factor	0.221
Rwork	0.219
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.814
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.380
Number of reflections	28305
<I/σ(I)>	13.96	2.57
Completeness [%]	100.0	100
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1