5A2H

Crystal Structure of Arabidopsis thaliana Calmodulin-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	77.15
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	24.455, 67.901, 111.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.960 - 2.270
R-factor	0.19318
Rwork	0.191
R-free	0.23822
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rfj
RMSD bond length	0.016
RMSD bond angle	1.667
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.200
High resolution limit [Å]	2.270	2.270
Rmerge	0.070	0.200
Number of reflections	8910
<I/σ(I)>	12.1	6.8
Completeness [%]	98.6	94.2
Redundancy	5.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2		MPD 60%, SODIUM ACETATE PH 4.2, CACL2 5MM