5A2F
Two membrane distal IgSF domains of CD166
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-04 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.320, 72.320, 105.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.090 - 1.860 |
R-factor | 0.2225 |
Rwork | 0.222 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cjj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.930 |
Data reduction software | XDS (VIA XIA2) |
Data scaling software | Aimless (VIA XIA2) |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.140 | 1.910 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.080 | 0.660 |
Number of reflections | 24110 | |
<I/σ(I)> | 20.1 | 3.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13 | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M SODIUM HEPES, PH 7.5, 25% PEG-2000 MME |