5A2B
Crystal Structure of Anoxybacillus Alpha-amylase Provides Insights into a New Glycosyl Hydrolase Subclass
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-10 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.013, 65.296, 126.708 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.350 - 1.850 |
| R-factor | 0.15656 |
| Rwork | 0.155 |
| R-free | 0.19273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e2o |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.896 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 43446 | |
| <I/σ(I)> | 15.7 | 1.8 |
| Completeness [%] | 94.5 | 83.6 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M CALCIUM ACETATE 0.1 M SODIUM CACODYLATE (PH 6.5) 18% (W/V) POLYETHYLENE GLYCOL 8, 000 20 MM MALTOSE |
