5A2A
Crystal Structure of Anoxybacillus Alpha-amylase Provides Insights into a New Glycosyl Hydrolase Subclass
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Collection date | 2014-07-10 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.429, 63.443, 122.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.900 |
| R-factor | 0.1799 |
| Rwork | 0.177 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e2o |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.232 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.420 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 34971 | |
| <I/σ(I)> | 13.3 | 2.5 |
| Completeness [%] | 98.1 | 93.4 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M CALCIUM ACETATE, 0.1 M SODIUM CACODYLATE (PH 6.5) 18% (W/V) POLYETHYLENE GLYCOL 8, 000 |
