5A24
Crystal structure of Dionain-1, the major endopeptidase in the Venus flytrap digestive juice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.850, 47.790, 57.790 |
| Unit cell angles | 90.00, 92.44, 90.00 |
Refinement procedure
| Resolution | 13.270 - 1.500 |
| R-factor | 0.1579 |
| Rwork | 0.158 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s4v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 1.700 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.030 | 0.090 |
| Number of reflections | 30734 | |
| <I/σ(I)> | 30.7 | 16 |
| Completeness [%] | 94.9 | 94.8 |
| Redundancy | 3.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | DI-POTASSIUM HYDROGEN PHOSPHATE 0.8 M, SODIUM DIHYDROGEN PHOSPHATE 0.9 M, LITHIUM SULFATE ANHYDROUS 0.2 M, CAPS 0.1 M, PH 10.5 |
