5A23
SdsA sulfatase triclinic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-13 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 86.900, 86.800, 129.030 |
Unit cell angles | 107.59, 102.96, 95.59 |
Refinement procedure
Resolution | 118.770 - 2.410 |
R-factor | 0.25624 |
Rwork | 0.253 |
R-free | 0.30701 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cg3 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.894 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.460 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.060 | 0.330 |
Number of reflections | 95678 | |
<I/σ(I)> | 15.34 | 1.65 |
Completeness [%] | 71.8 | 59.7 |
Redundancy | 2.97 | 1.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 10% PEG 4000, 10% ISOPROPANOL, 100MM LICL, 100 MM SODIUM CITRATE PH 6. |