5A23
SdsA sulfatase triclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.900, 86.800, 129.030 |
| Unit cell angles | 107.59, 102.96, 95.59 |
Refinement procedure
| Resolution | 118.770 - 2.410 |
| R-factor | 0.25624 |
| Rwork | 0.253 |
| R-free | 0.30701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cg3 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.894 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 95678 | |
| <I/σ(I)> | 15.34 | 1.65 |
| Completeness [%] | 71.8 | 59.7 |
| Redundancy | 2.97 | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% PEG 4000, 10% ISOPROPANOL, 100MM LICL, 100 MM SODIUM CITRATE PH 6. |
