5A21
Structure of bacteriophage SPP1 head-to-tail interface without DNA and tape measure protein
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 400 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.2 |
| Software | Flex-EM,MODELLER,UCSF Chimera,VEDA,EMAN,IMAGIC,SPIDER |
| Symmetry | C6 |
