5A1K
Crystal structure of calcium-free human adseverin domains A1-A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-12 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.410, 65.250, 105.190 |
Unit cell angles | 90.00, 118.27, 90.00 |
Refinement procedure
Resolution | 59.630 - 2.900 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.24780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1D0N RESIDUES 27-370 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.600 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.150 | 1.330 |
Number of reflections | 17766 | |
<I/σ(I)> | 10.4 | 1.5 |
Completeness [%] | 98.0 | 95 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20 % POLYETHYLENE GLYCOL 3350, 100 MM BIS-TRIS PROPANE PH 7.5 |