5A1H
Crystal structure of human Spindlin3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2015-02-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.580, 129.400, 129.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.140 - 2.000 |
| R-factor | 0.17358 |
| Rwork | 0.172 |
| R-free | 0.21244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ns2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.110 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | 0.680 |
| Number of reflections | 67153 | |
| <I/σ(I)> | 11.7 | 3.1 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 7.6 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M TRIS PH 8.4, 38% PEG3350 |
