5A16

Crystal structure of Fab4201 raised against Human Erythrocyte Anion Exchanger 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-28
Detector	MARRESEARCH
Spacegroup name	P 41
Unit cell lengths	82.190, 82.190, 270.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.514 - 2.500
R-factor	0.2149
Rwork	0.213
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r8s
RMSD bond length	0.004
RMSD bond angle	0.933
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.130	0.480
Number of reflections	61639
<I/σ(I)>	9	2.6
Completeness [%]	99.9	100
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				12% (W/V) PEG20000, 0.1% NA ACETATE (PH5.3)