5A12
Crystal structure of Chlorite Dismutase from Magnetospirillum sp. in complex with azide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2013-12-12 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.234, 133.823, 77.622 |
Unit cell angles | 90.00, 113.03, 90.00 |
Refinement procedure
Resolution | 71.440 - 1.400 |
R-factor | 0.11994 |
Rwork | 0.118 |
R-free | 0.15434 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q08 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.519 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.840 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.020 | 0.740 |
Number of reflections | 268333 | |
<I/σ(I)> | 11.4 | 1.4 |
Completeness [%] | 98.4 | 94.2 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 16% PEG 6000, 0.01M SODIUM CITRATE |