5A0P
Apo-structure of metalloprotease Zmp1 from Clostridium difficile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.169, 71.769, 117.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.530 - 1.398 |
| R-factor | 0.1573 |
| Rwork | 0.156 |
| R-free | 0.18170 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.022 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.520 |
| Number of reflections | 71874 | |
| <I/σ(I)> | 15.66 | 2.48 |
| Completeness [%] | 98.2 | 95.6 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 2.1 M AMMONIUM PHOSPHATE, 0.1 M TRIS PH 8.0 AT 293 K |
