5A0N
N-terminal thioester domain of protein F2 like fibronectin-binding protein from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-24 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.860, 59.860, 121.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.710 - 1.300 |
| R-factor | 0.12087 |
| Rwork | 0.119 |
| R-free | 0.15140 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.670 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.300 |
| Number of reflections | 51670 | |
| <I/σ(I)> | 33.8 | 7.4 |
| Completeness [%] | 93.6 | 62.7 |
| Redundancy | 23.9 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.02 M SODIUM L-GLUTAMATE, 0.02 M DL-ALANINE, 0.02 M GLYCINE, 0.02 M DL-LYSINE, 0.02 M DL-SERINE, 0.1 M MES/IMIDAZOLE PH 6.5, 10% PEG 4000, 20% GLYCEROL |
