5A0M

THE CRYSTAL STRUCTURE OF I-SCEI IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF MN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 21
Unit cell lengths	80.113, 80.565, 130.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.897 - 2.900
R-factor	0.1926
Rwork	0.186
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB NETRY 2VS8
RMSD bond length	0.011
RMSD bond angle	1.253
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.620	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.100	0.580
Number of reflections	36541
<I/σ(I)>	12.1	2.7
Completeness [%]	100.0	99.2
Redundancy	8.1	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1