5A0L
N-terminal thioester domain of fibronectin-binding protein SfbI from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 41 |
| Unit cell lengths | 165.120, 165.120, 42.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.220 - 1.350 |
| R-factor | 0.13173 |
| Rwork | 0.131 |
| R-free | 0.15269 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.528 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.220 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.050 | 0.750 |
| Number of reflections | 126127 | |
| <I/σ(I)> | 18.7 | 2.7 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 9.2 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M ZINC ACETATE, 0.1 M HEPES PH 7.5, 14% PEG 8000 |
