5A01
O-GlcNAc transferase from Drososphila melanogaster
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 32 |
Unit cell lengths | 160.946, 160.946, 77.189 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 - 2.660 |
R-factor | 0.22672 |
Rwork | 0.225 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pe4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.259 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.750 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.120 | 0.920 |
Number of reflections | 63989 | |
<I/σ(I)> | 8.8 | 1.5 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 5.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | CONDITION 70 OF THE MOLECULAR DIMENSIONS MORPHEUS SCREEN 0.12 M MIX OF D-GLUCOSE, D-MANNOSE, D-GALACTOSE, L-FUCOSE, D-XYLOSE, N-ACETYL-D-GLUCOSAMINE, 30% PEG8000/ETHYLENE GLYCEROL AND 0.1 M TRIS-BICINE PH 8.5 |