5ZZW

Proteobacterial origin of protein arginine methylation and regulation of Complex I assembly by MidA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	173
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	72.950, 103.540, 192.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.540 - 2.600
R-factor	0.25001
Rwork	0.248
R-free	0.27989
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ztz
RMSD bond length	0.007
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0216)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.540	2.693
High resolution limit [Å]	2.590	2.590
Rmerge	0.186
Number of reflections	43310	4454
<I/σ(I)>	4.9
Completeness [%]	97.7
Redundancy	6.2
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M potassium sodium tartrate tetrahydrate, 0.1M BIS-TRIS pH 6.5, 10% w/v polyethylene glycol 10000