5ZYS

Structure of Nephrin/MAGI1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	0.97853
Spacegroup name	I 2 3
Unit cell lengths	93.731, 93.731, 93.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.865 - 1.780
R-factor	0.1822
Rwork	0.181
R-free	0.20440
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.173
Data reduction software	d*TREK
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.780	4.830	1.780
Rmerge	0.069	0.057	0.172
Rmeas	0.079	0.064	0.202
Rpim	0.037	0.030	0.102
Total number of observations	51381
Number of reflections	13086	668	683
<I/σ(I)>	6.2
Completeness [%]	98.1	92.8	99.9
Redundancy	3.9	4	3.6
CC(1/2)		0.994	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.5M sodium chloride , 0.01M magnesium chloride hexahydrate , 0.01Mhexadecyltrimethylammonium bromide