5ZYF
Crystal structure of Streptococcus pyogenes type II-A Cas2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.771, 75.292, 145.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.448 - 1.760 |
R-factor | 0.1984 |
Rwork | 0.197 |
R-free | 0.23360 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.018 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.071 | 0.547 |
Rmeas | 0.077 | 0.594 |
Rpim | 0.029 | 0.228 |
Number of reflections | 56417 | 5499 |
<I/σ(I)> | 22.45 | 3.417 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 7.2 | 6.5 |
CC(1/2) | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 22.0% (w/v) PEG 4000, 325 mM ammonium sulfate, 0.1 M sodium acetate pH 4.6 |