5ZXU

Crystal structure of CurA in complex with NADPH from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 41 21 2
Unit cell lengths	90.137, 90.137, 105.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.050 - 2.200
R-factor	0.2027
Rwork	0.201
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zxn
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.050	2.320
High resolution limit [Å]	2.200	2.200
Rmeas	0.073	1.073
Number of reflections	22720	3257
<I/σ(I)>	2.2	0.8
Completeness [%]	99.9	100
Redundancy	11.4	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	sodium chloride, MES, PEG 8000