5ZXG
Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, ligand-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-15 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.250, 179.798, 63.412 |
| Unit cell angles | 90.00, 113.61, 90.00 |
Refinement procedure
| Resolution | 89.900 - 2.400 |
| R-factor | 0.19103 |
| Rwork | 0.188 |
| R-free | 0.24812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0h |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.855 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.900 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.048 | 0.096 |
| Number of reflections | 35608 | |
| <I/σ(I)> | 0.232 | 0.131 |
| Completeness [%] | 92.2 | 92.1 |
| Redundancy | 3.2 | 3.1 |
| CC(1/2) | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M sodium citrate, 0.22 M Ammonium sulfate, 30% (w/v) PEG4000 |
