5ZXE

Structure of a consensus sequence derived from the FGF family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9660
Spacegroup name	P 2 21 21
Unit cell lengths	35.718, 45.910, 90.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.435 - 1.300
R-factor	0.1722
Rwork	0.171
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fgf
RMSD bond length	0.005
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.910	45.910	1.260
High resolution limit [Å]	1.240	6.790	1.240
Rmerge	0.061	0.028	0.948
Rmeas	0.068	0.031	1.072
Rpim	0.030	0.014	0.484
Total number of observations	217407
Number of reflections	42829	326	2048
<I/σ(I)>	12
Completeness [%]	99.2	99.2	98.3
Redundancy	5.1	4.8	4.4
CC(1/2)	0.999	0.999	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	PEG 8000, Ammonium sulphate