5ZX3
Mycobacterium tuberculosis RNA polymerase holoenzyme with ECF sigma factor sigma H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.572, 159.790, 131.437 |
| Unit cell angles | 90.00, 118.72, 90.00 |
Refinement procedure
| Resolution | 41.638 - 2.751 |
| R-factor | 0.2185 |
| Rwork | 0.218 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tw1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.565 |
| Data reduction software | HKL-2000 (v716) |
| Data scaling software | HKL-2000 (v716) |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.078 | 0.039 | 0.893 |
| Rmeas | 0.086 | 0.044 | 1.037 |
| Rpim | 0.036 | 0.018 | 0.515 |
| Total number of observations | 650968 | ||
| Number of reflections | 119837 | 6206 | 5125 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 98.2 | 99.4 | 84.3 |
| Redundancy | 5.4 | 5.6 | 3.5 |
| CC(1/2) | 0.998 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M NaAc, 0.1 M sodium citrate pH5.5, 10% PEG4000 |
