5ZWS

Crystal structure of apo-acyl carrier protein from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541780
Spacegroup name	P 1 21 1
Unit cell lengths	30.069, 47.442, 61.128
Unit cell angles	90.00, 92.81, 90.00

Refinement procedure

Resolution	37.462 - 2.000
R-factor	0.2529
Rwork	0.252
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x3o
RMSD bond length	0.003
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.167	0.526
Rmeas	0.185
Rpim	0.079	0.257
Number of reflections	11636
<I/σ(I)>	11.2
Completeness [%]	99.0
Redundancy	5.3
CC(1/2)		0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	294	0.1M Bis-Tris pH 5.5, 0.2M sodium chloride, 25% w/v polyethelene glycol 3350