5ZW4

Crystal structure of tRNA bound TrmR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	64.982, 64.982, 126.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.010 - 1.700
R-factor	0.1943
Rwork	0.193
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gpy
RMSD bond length	0.009
RMSD bond angle	2.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.090	0.065
Rmeas	0.093	0.067
Rpim	0.024	0.016	0.677
Number of reflections	32851	1870	1400
<I/σ(I)>	10.2
Completeness [%]	94.1	97.3	81.4
Redundancy	17.5	17	9.5
CC(1/2)		0.997	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4 M Ammonium phosphate monobasic