5ZVC
P domain of GII.13 norovirus capsid complexed with Lewis A trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-04-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.230, 82.618, 116.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.15677 |
| Rwork | 0.154 |
| R-free | 0.20097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zv9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.157 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.130 | 0.474 |
| Number of reflections | 57510 | 1898 |
| <I/σ(I)> | 19.3 | 3.5 |
| Completeness [%] | 94.5 | 63.2 |
| Redundancy | 6.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M succinic acid (pH 7.0), 15% (w/v) polyethylene glycol (PEG) 3350 |
