5ZVA

APOBEC3F Chimeric Catalytic Domain in Complex with DNA(dC9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-03
Detector	MAR CCD 130 mm
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	61.312, 91.304, 91.354
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 2.300
R-factor	0.164
Rwork	0.161
R-free	0.20800
Structure solution method	SAD
Data reduction software	ADDREF
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.121	0.619
Number of reflections	23245
<I/σ(I)>	14
Completeness [%]	98.5
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.05M sodium cacodylate, pH 6.5, and 30%(v/v) 2-methyl-2,4-pentanediol(MPD)