5ZUU
Crystal structure of AtCPSF30 YTH domain in complex with 10mer m6A-modified RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.108, 74.293, 151.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.20223 |
| Rwork | 0.199 |
| R-free | 0.25577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wqn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.885 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.145 | |
| Number of reflections | 57240 | 5582 |
| <I/σ(I)> | 15.25 | |
| Completeness [%] | 99.6 | |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH 7.5, 20% PEG 10,000 |
