5ZUM
Structure of dipeptidyl-peptidase III from Corallococcus sp. strain EGB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.141, 77.850, 228.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.17634 |
| Rwork | 0.174 |
| R-free | 0.22361 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.515 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 9.870 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 82472 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.59 | 293 | 24%(w/v) PEG 3350, 0.1M sodium cacodylate acetate trihydrate pH 4.59, 0.02M TCEP hydrochioride |
