5ZUG

Structure of the bacterial acetate channel SatP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9709
Spacegroup name	P 32
Unit cell lengths	99.504, 99.504, 137.824
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.533 - 2.802
R-factor	0.227
Rwork	0.225
R-free	0.26690
RMSD bond length	0.010
RMSD bond angle	1.229
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.533	2.900
High resolution limit [Å]	2.802	2.802
Rmerge	0.154	0.605
Number of reflections	37419
<I/σ(I)>	3.8
Completeness [%]	99.8
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		297	0.05M Glycine PH 9.8, 27% PEG 300, 0.1M NaCl