5ZUE

GTP-bound, double-stranded, curved FtsZ protofilament structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9796
Spacegroup name	P 65 2 2
Unit cell lengths	100.526, 100.526, 138.341
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.500 - 2.700
R-factor	0.211
Rwork	0.210
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.520	2.780
High resolution limit [Å]	2.700	2.700
Number of reflections	11803
<I/σ(I)>	44.1
Completeness [%]	99.5
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	10mM GTP, 1M sodium citrate and 0.1M imidizole, pH 8.0.