5ZU4

Crystal structure of Lamprey immune protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	105.049, 105.049, 168.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.080 - 2.390
R-factor	0.1953
Rwork	0.193
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nzo
RMSD bond length	0.013
RMSD bond angle	1.658
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.080	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	41118
<I/σ(I)>	28.3
Completeness [%]	99.9
Redundancy	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289	1,4-Dioxane, Polyethylene glycol 20000