5ZU0

Proteobacterial origin of protein arginine methylation and regulation of Complex I assembly by MidA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	173
Detector technology	CCD
Collection date	2012-05-11
Detector	RAYONIX MX300HE
Wavelength(s)	0.9870, 0.9794, 0.9642
Spacegroup name	P 21 21 21
Unit cell lengths	72.828, 105.163, 201.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.610 - 2.760
R-factor	0.20186
Rwork	0.200
R-free	0.23583
RMSD bond length	0.010
RMSD bond angle	1.528
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.610	2.860
High resolution limit [Å]	2.760	2.760
Number of reflections	39759	3811
<I/σ(I)>	12.64
Completeness [%]	98.1
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M potassium sodium tartrate tetrahydrate, 0.1M BIS-TRIS pH 6.5, 10% w/v polyethylene glycol 10000