5ZTL

Non-cryogenic structure of light-driven chloride pump having an NTQ motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	294
Detector technology	PIXEL
Collection date	2017-12-14
Detector	CS-PAD CXI-2
Wavelength(s)	1.3
Spacegroup name	C 1 2 1
Unit cell lengths	103.360, 50.090, 69.400
Unit cell angles	90.00, 109.65, 90.00

Refinement procedure

Resolution	18.520 - 1.850
R-factor	0.242
Rwork	0.240
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g28
RMSD bond length	0.010
RMSD bond angle	0.970
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.520	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	28973
<I/σ(I)>	4
Completeness [%]	100.0
Redundancy	52.6
CC(1/2)	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	294	30% PEG 500 DME, 0.1M sodium chloride, 0.1M MES (pH 6.0)